Test methods

 

 

The EURLP’s diagnostic activities are performed in accordance with ISO/IEC 17025. The lack of international standards for the diagnosis of foodborne parasitic diseases is a major constraint in implementing a quality assurance system. To date, few diagnostic methods have been standardized at European or international level (ISO 18743:2015. Microbiology of the food chain — Detection of Trichinella larvae in meat by artificial digestion method; ISO18744:2016. Microbiology of the food chain — Detection and enumeration of Cryptosporidium and Giardia in fresh leafy green vegetables and berry fruits). Thus there is a need to validate both commercial kits and in-house developed tests. EURLP staff, on the basis of long-lasting scientific experience, developed new tests and reagents for detecting parasitic pathogens, and contributed to work up international guidelines.
EURLP developed and validated the following diagnostic methods:

Detection of anti-Trichinella antibodies in swine serum by indirect ELISA  (MI-01)

Identification of Trichinella muscle larvae at species level by Multiplex PCR  (MI-02)

Detection of anti-Trichinella antibodies in human serum by indirect ELISA (MI-03)

Identification at species level of parasites of the family Anisakidae by PCR/RFLP (MI-04)

Identification of Echinococcus granulosus complex at genotype/species level from hydatid cysts by PCR and sequencing  (MI-05)

Identification at species level of Cryptosporidium oocysts by PCR/RFLP (MI-06)

Detection of anti-Opisthorchis antibodies in human sera (MI-07)

Identification of Opisthorchis spp eggs by PCR (MI-08)

Identification at assemblage level of Giardia duodenalis cysts by PCR/RFLP (MI-09)

Identification of Anisakidae larvae at the species level by multiplex PCR (MI-10)

Identification of the assemblages A and B of Giardia duodenalis (MI-11)

Identification of Toxoplasma gondii DNA in food matrices (fresh or processed meat) by LAMP (MI-12)

Identification of Trichinella spp. proteins recognized by specific IgG in serum of infected pigs by western blotting  (MI-13)

Identification of Toxoplasma gondii DNA in leafy green vegetable by LAMP  (MI-14)

 

The methods listed, together with the magnetic stirrer method for pooled sample digestion for the detection of Trichinella in fresh meat (Commission Regulation No 2015/1375, see Section “Documents – EU Regulations”), were accredited by ACCREDIA, the italian accreditation body, according to the ISO/IEC 17025:2005 international standard.
The accreditation is submitted to a convention and recognises the technical competence of the laboratory to perform specific analytical methods. Nevertheless, the accreditation body doesn’t assume any responsability related to the results of the assays or their interpretation.
All methods are available as part of the services that EURLP provides to NRLs. Other public institutions wishing to have access to the EURLP services, are requested to send by e-mail an application to EURLP.
Before sending the samples to be tested, please download the Test Request Form from the “old” EURLP web site http://old.iss.it/crlp/index.php?lang=2&anno=2018&tipo=33, fill it and send it by fax (+39 0649903561), e-mail to EURLP.

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